Date Published: 5 June 2013

Malaria parasites stick to blood vessel linings through a group of parasite proteins called PfEMP1

Recent revelations about how parasites attach to blood vessels could lead to new techniques to combat malaria - according to research conducted by experts based in Oxford, Copenhagen (Denmark), Seattle (USA) and Dar es salaam (Tanzania).

The researchers identified how malaria parasites growing inside red blood cells stick to the sides of blood vessels in severe cases of malaria. It is hoped that this discovery might advance the development of vaccines or drugs to combat severe malaria by stopping the parasites attaching to blood vessels.

Although it has been known for over 100 years that red blood cells infected with malaria parasites can kill their host by sticking to the sides of blood vessels, the binding mechanism associated with the most lethal forms of malaria was unknown. In the recent study, which has been published in the journal Nature, it is shown that the parasite binds a protein in blood vessel walls called endothelial protein C (EPCR), which is involved with regulating blood coagulation and the inflammatory response.

Malaria parasites grow in red blood cells and stick to the endothelial lining of blood vessels through a large family of parasite proteins called PfEMP1. In this way, the parasite avoids being carried with the blood to the spleen, where it would otherwise be destroyed. One of the most aggressive forms of malaria parasite binds in brain blood vessels, causing a disease called cerebral malaria. In 2012, three groups of researchers, including the teams at Copenhagen University and Seattle Biomedical Research Institute, showed that a specific type of PfEMP1 protein was responsible for cerebral binding and other severe forms of malaria infection. However, until recently, the receptor to which it binds was not known.

" The first big challenge was to generate a full-length PfEMP1 protein in the laboratory," said Assistant Professor Louise Turner at the University of Copenhagen.
" Next, we utilized a new technology developed by Retrogenix LTD in the United Kingdom to examine which of over 2,500 human proteins this PfEMP1 protein could bind to."

Of the 2,500 proteins screened, a receptor called endothelial protein C (EPCR) was the single solid hit.

"A lot of work then went into confirming this binding in the lab and not least to show that parasites from non-immune children with severe malaria symptoms in Tanzania often bound EPCR," she continued.

" It was a true eureka moment," said Assistant Professor Thomas Lavstsen.
" Under normal conditions, ECPR plays a crucial role in regulating blood clotting, inflammation, cell death and the permeability of blood vessels. The discovery that parasites bind and interfere with this receptor´s normal function may help us explain why severe symptoms of malaria develop."

Malaria parasites disrupt the important functions of blood vessels. Severe malaria symptoms such as cerebral malaria often result in minor blood clots in the brain. One of the body´s responses to malaria infection is to produce inflammatory cytokines, but too much inflammation is dangerous, explained Professor Joseph Smith, stating that

" ECPR and a factor in the blood called protein C act as a 'brake' on blood coagulation and endothelial cell inflammation and also enhance the viability and integrity of blood vessels, but when the malaria parasites use PfEMP1 to bind EPCR, they may interfere with the normal function of EPCR, and thus the binding can be the catalyst for the violent reaction."
"Investigating this question is the next step to learn about how malaria parasites cause disease."

According to the researchers, the discovery that malaria parasites bind EPCR might advance vaccine and drug interventions to treat severe malaria.

Dr. Matthew Higgins from the University of Oxford explained:

" Now that we know the pair of proteins involved, we can begin zooming further in to reveal the molecular details of how malaria parasites grab onto the sides of blood vessels. We want to know exactly which bits of the parasite protein are needed to bind to the receptor in the blood vessel wall. Then, we can aim to design vaccines or drugs to prevent this binding."

Vaccine research will also benefit immediately from the discovery, since scientists can already now test the effectiveness of different vaccine candidates at preventing PfEMP1 from binding endothelial protein C (EPCR).

" Over the last decade, we have come to appreciate that specific PfEMP1 proteins are associated with different severe forms of malaria," explained Professor Thor Theander at the University of Copenhagen.
" Together with The National Institute for Medical Research Tanzania, we are in the process of preparing phase I trials for a vaccine to prevent parasite binding in the placenta and malaria during pregnancy," he said.

This new discovery holds the potential for also developing a vaccine to reduce the heavy burden malaria disease inflicts on children.

" It will be a long haul, but with these results, we can get started right away," added Prof. Theander.

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